Artemisia vulgaris anthelmintic activities to ova and adult stages of Fasciola gigantica in vitro

Background and Aim: Fasciolosis due to Fasciola gigantica is endemic tropical countries hepatica in temperate climates, highly detrimental livestock known as foodborne zoonotic diseases. The strategic control of the disease mainly use chemical anthelmintic. This study aimed evaluate anthelmintic properties Artemisia vulgaris extract on ova adult stages F. gigantica. Materials Methods: Samples were collected from Ampel Abbatoir, Boyolali District, Central Java, Indonesia. 20-gallbladders cattle which naturally infected with 270 living worms used this study. ovicidal assay was performed by incubating A. different concentrations, that is, 5%, 2.5%, 1.25% for 5, 9, 11, 14, 16 days. efficacies evaluated quantification degeneration during developmental time points egg-hatch assay. flukicidal effects observed mortality 10, 20, 40, 80, 160, 320, 640 min incubations followed scanning electron microscopy surface morphology histology fluke’s transversal sections. Results: concentration 5% showed strongest activities. percentage hatching day at concentrations 3.33%, 6.67%, 16.67%. These hatch a significant reduction (p < 0.001) compared untreated control. effect 20%, rate reaching 66.67% 40 incubation time. worms, including spine, tegument, acetabulum, intestine, vitelline follicles, disintegrated. Conclusion: results has potential active compounds remained necessary be elucidated further its modes action would interesting predicted molecular docking modeling. Keywords: anthelmintic, vulgaris, gigantica, flukicidal, ovicidal.